Toti has been under house arrest since May 7 in relation to a corruption probe regarding alleged bribes in which cash for an election campaign was exchanged for favours.
One favour included the extension of a port terminal concession.
The centre-right politician has said he is innocent and will be able to show his management of the northwestern Italian region was clean.
The new accusation concerns election ads projected onto a big screen in Genoa’s Terrazza Colombo rooftop alleged funded via undeclared money from the Esselunga supermarket chain.
Toti’s former right-hand man Matteo Cozzani and former senator Maurizio Rossi, owner of the Primocanale TV channel, and former Esselunga board member Francesco Moncada are also under investigation, the sources said.
ANSA