The proposed entrance fee will reportedly be introduced from May 2, 2018.
The accord was signed in the presence of the Italian minister of culture and tourism, Dario Franceschini, and the Vicar General of Rome, Angelo De Donatis.
The culture ministry said the entrance fee will be used to “better valorise and safeguard the monument, as well as fund maintenance costs and guarantee better security during visits.”
Entrance will remain free for those attending religious services in the iconic ancient Roman monument, now also serves as a church.
Rome’s Deputy Mayor Luca Bergamo said he would ask for the “sad” measure to be revoked.
Mr Bergamo said if the measure is introduced, he hopes that the ministry will set aside funds to cover the cost of monitoring the queues which will inevitably form outside the historical site.
Originally a temple for all the Roman gods, the Pantheon is one of Rome's most iconic monuments.
Built in 113 – 125 AD, it houses the tomb of Renaissance painter Raphael.
With ANSA