The 5-Star Movement (M5S) presented the motion after she reported to parliament earlier this month over allegations regarding her business activities.
The other main opposition group, the centre-left Democratic Party (PD), has said it will back the no-confidence motion.
Santanchè is reportedly under investigation although she says she has not received notification of the probe.
She denies any wrongdoing.
The minister, a leading member of Prime Minister Giorgia Meloni’s Brothers of Italy (FdI) party, has been facing calls to quit since investigative journalism program Report on Rai 3 reported that businesses linked to her allegedly failed to pay suppliers and dismissed workers without giving them redundancy payments, as well as allegedly improperly receiving COVID aid.
ANSA