The Giro will take place from Saturday, May 8 to Sunday, May 30, with the full race route expected to be announced later this month.
Filippo Ganna is the favourite to win the opening nine-kilometre individual time trial just as he did in 2020 when the Grande Partenza was held in Palermo.
Egan Bernal and Vincenzo Nibali are among the overall contenders for the maglia rosa.
Race organiser RCS Sport on Thursday confirmed Turin as the host of the start.
“On the 160th anniversary of the unification of Italy, we could only choose Piedmont and Turin, the first Italian capital, for the Grande Partenza of the Giro's 104th edition,” Paolo Bellino, CEO of RCS Sport, said.
Stage 2 to Novara will offer the sprinters an early chance of victory, while Stage 3 will also be held in the Piedmont region, starting in Biella and finishing in Canale, south of Turin.
The Giro will visit the region again late in the race, with stage 19 set to finish at altitude at Alpe di Mera, while the following day’s stage will start from Ganna’s hometown of Verbania.
The final stage is expected to be a time trial to the centre of Milan, giving Ganna another chance of a stage victory.
After the early Piedmont stages, the route is expected to head south via the central Apennines and Emilia Romagna and then onto Puglia, before returning north via the Tuscan strade bianche and then east to climb the Zoncolan.
The Giro last started in Turin in 2011, to celebrate the 150th anniversary of the nation’s unification.