Fatalities since Sunday included the first death in the central Marche region: an 88-year-old man who, like most of the deceased in Italy, had pre-existing health conditions.
Previously, all of the deaths have been in the worst-hit northern regions of Lombardy, Emilia-Romagna and Veneto.
The civil protection agency said three more had died in Emilia-Romagna, all of whom were either elderly or had serious pre-existing medical conditions.
There were more fatalities in Lombardy, the epicentre of the outbreak where the majority of the deaths have occurred.
Around 166 people remained in intensive care, while 149 patients had recovered since the epidemic hit Italy.
Those who have died are all Italian citizens.
Most of the victims were elderly, many over the age of 80, had underlying health conditions including cancer, and were already in hospital when the virus was detected.
Italy is Europe’s worst-affected country.
It has carried out over 23,300 tests so far, largely in the wealthy north, where 11 towns have been placed under lockdown.
Tourist hotpots including the Duomo in Milan reopened their doors to visitors Monday, but access was limited to avoid overcrowding in a bid to prevent the disease from spreading.
The first confirmed cases were reported in Rome and the southern regions of Molise and Sardinia on Monday, areas which had been unaffected by the outbreak until this week.
Italian health officials said on Monday they were “optimistic” that measures currently in place would be enough to contain the outbreak.
The next seven days will be “decisive” in stopping the spread, said the head of Italy’s National Health Institute Silvio Brusaferro.
“At the end of the week we will understand if and how much the containment measures put in place have slowed the epidemic,” he told a press conference on Monday.
“We expect positive results, I am optimistic.
“We ask all citizens for collaboration; their help is important for breaking the chain of infections.”
He said the cases being confirmed now were likely to be people who were infected before the measures were put in place.
Researchers last week said the virus could have been “circulating for weeks” undetected in Italy.
Italy has seen a huge surge in the number of cases over the last 10 days, with cases doubling in 48 hours over the weekend.
Italy has also been a point of contagion with many cases in other countries involving people who returned home after travelling in infection-hit areas of northern Italy.
The economy has been badly damaged by the outbreak, with Ryanair announcing on Monday that it was scaling back services to Italy after a significant drop in bookings.
BA and easyJet have also cancelled some flights; American Airlines has suspended flights to Milan until April 24.
The Italian government is seeking support from Brussels to approve a €3.6 billion package to help businesses withstand the economic fallout.
This follows €900 million worth of financial relief measures for the 11 northern towns that are still under lockdown.
