Those killed in the blast were employee and caretaker of the Accademia, Angela Fiume, 36, her policeman husband Fabrizio Nencioni, 39, their daughters Caterina Nencioni, 50 days old, and Nadia Nencioni, 9, and Dario Capolicchio, 22, a junior architecture student at the university.
Forty-eight people were also injured by the blast.
Thirty years after the tragedy, authorities and hundreds of people paid tribute to them with the laying of a wreath at the site of the explosion, at the exact time when the attack took place at 1:04am on 27 May, 1993.
Among those present were the Mayor of Florence Dario Nardella, the President of the Region of Tuscany Eugenio Giani and the Undersecretary for Justice Andrea Delmastro.
The bombing was part of a wave of Sicilian Mafia bombings in 1993 aimed at hitting back against a State crackdown following the bombing deaths of magistrates Giovanni Falcone and Paolo Borsellino a year earlier, in May and July 1992.
The Mafia bombings in 1993 killed 10 people and damaged art and religious sites in Rome, Florence and Milan.
ANSA