Turin giants Juventus, whose president Andrea Agnelli was one of the biggest advocates of the controversial project, reluctantly conceded that the withdrawal of most of the 12 founding teams made the realisation of the breakaway league impossible.
In an interview given before the withdrawal of England’s “big six”, Agnelli had insisted the league would go ahead.
“[The project] has a 100 per cent chance of success,” he said.
Agnelli said he remained convinced European football needed to change and he had no regrets about the way the breakaway attempt was made.
“Football is no longer a game but an industrial sector and it needs stability,” he added.
Like Juventus, AC Milan did not formally withdraw but said they were giving up on the league following widespread outrage and protests.
“The voices and the concerns of fans around the world have clearly been expressed about the Super League, and AC Milan must be sensitive to the voice of those who love this wonderful sport,” the seven-time European champions said in a statement.
“Change is not always easy, but evolution is necessary for progress, and the structures of European football have evolved and changed over the decades.
“We will continue to work hard to deliver a sustainable model for football.”
Inter Milan also confirmed they were “no longer part of the Super League project”.
“Inter believes that football, like every sector of activity, must have an interest in constantly improving its competitions, to keep on exciting fans of all ages around the world, within a framework of financial sustainability,” the club said.
Spanish giants Barcelona and Real Madrid officially remain the only teams left in the project three days after its announcement.
The original plan was to create a rival to the Champions League featuring 20 teams, 15 of which would be permanent members and would not need to qualify.
Many argued the competition would be the “death of football”.
Italian daily Fatto Quotidiano described the proposal as “a league where only the rich play against the rich, to become even richer”.