The move passed its final legislative step with a vote in the European Parliament on Tuesday.
The intention of the law is to speed up the broad switch to electric vehicles and combat climate change.
The landmark rules will require that by 2035 car makers must achieve a 100 per cent cut in CO2 emissions from new cars sold, which would make it impossible to sell new fossil fuel-powered vehicles in Europe's 27-country bloc.
"Italy is behind with the transition (to electric) of the car sector and we must accelerate with investments," Urso told Rai radio.
"But the time frames and procedures that Europe is imposing on us do not match the European reality and, above all, they don't match the Italian one.
"We cannot address reality with an ideological, partisan vision, which is what seems to emerge from the European institutions".
Tajani, who is also deputy premier, echoed those sentiments, saying the move was a "serious mistake".
"I am a great supporter of electric cars, but ambitious targets have to be achieved in reality, not just on paper," Tajani told Rai television.
"Italy will forward a counter-proposal of limiting the reduction to 90 per cent, giving industries the chance to adapt.
"It is necessary to combat climate change, but this means setting achievable targets".
Deputy Premier and Transport and Infrastructure Minister Matteo Salvini has also decried the ban.
"It is a crazy, disconcerting decision that goes against Italian and European industries and workers and in favour of Chinese businesses," Salvini said on Tuesday.
ANSA
