Maria Laura Satta, 71, and Luigi Scassellati, 72, from the northern Italian city of Cremona, were attacked with a machete at their villa in Kikambala, a tourist resort near Mombasa.
Scassellati is reportedly in a stable condition and receiving hospital treatment in the Kenyan port city.
The couple are the owners of Seted, a company specialising in office equipment.
They were reportedly found in the villa by a friend.
Working in collaboration with the foreign ministry, Rome prosecutors have prepared international warrants to obtain evidence for the investigation.
With ANSA