The museums have been closed since November 5, due to coronavirus restrictions imposed across Italy.
The museums will open their doors to the public from Monday to Saturday, but visitors must pre-book tickets and will be given timed entry slots.
Curators used the closure to carry out maintenance and refurbishment works, including careful dusting of 15th-century frescoes in the Sistine Chapel, which normally attracts six million visitors a year.
Most museums and archaeological sites in Italy will reopen on Monday as the country eases restrictions, reclassifying all but five regions from medium-risk “orange zones” to lower-risk “yellow zones”.
Rome’s Colosseum and the Forum will also reopen on Monday.
The relaxing of restrictions also allows bars and restaurants to reopen during the day.
Italy currently has a complete ban in place on non-essential travel, including for tourism, between all regions regardless of their colour under the country’s three-tiered system of restrictions.
The entire country also remains subject to 10:00 pm to 5:00 am curfew, while bars and restaurants must end seated table service at 6:00 pm in yellow zones and cannot open at all in Italy’s remaining orange zones.
