Sunday’s Serie A match was temporarily halted and a stadium announcement made during the game urging fans not to sing discriminatory songs to Balotelli.
Brescia was leading 1-0 at the time following a goal scored by Balotelli, who had already been subjected to racial abuse earlier in the match.
Balotelli, 29, later said the spectators involved should be ashamed of themselves.
The striker posted a video of his goal on Instagram after the match with the caption: “Lazio fans who were at the stadium today SHAME ON YOU!”
Serie A judge Gerardo Mastrandrea requested that further details on the case be handled by the Italian soccer federation prosecutor to determine exactly where in the stadium the chants came from, and to what extent Lazio collaborates in terms of identifying those responsible, before possible further punishment.
Balotelli was targeted by Lazio fans for abuse all match with other offensive chants, that weren’t specifically racist, against him and his family.
“As has always happened in the past, Lazio dissociates itself in the most exhaustive way possible from the discriminatory behavior carried out by a very small minority of fans during the match against Brescia,” Lazio said in a statement following the incident.
“The club once again reiterates its condemnation of such unjustified misconduct and confirms its intent to prosecute those who in fact betray their sporting passion, causing serious damage to the image of the club.”
Balotelli was also the target of racial abuse earlier this season when he threatened to walk off the field because of racist chants directed at him by Hellas Verona fans during a match in November.
Racism has been a problem all season in Italy with offensive chants also aimed at Romelu Lukaku, Franck Kessie, Dalbert Henrique, Miralem Pjanic, Ronaldo Vieira and Kalidou Koulibaly.
Last month, all 20 of Italy’s Serie A clubs signed a joint open letter to fans condemning racism in stadiums.
The letter said Serie A intends to deliver a “comprehensive and robust” anti-racism policy, stricter new laws and regulations and a plan for educating those within the game about the scourge of racism.