The 82-year-old Godfather II, Raging Bull and Goodfellas star thanked the city at a ceremony on the Capitol.
De Niro’s artist father had Italian and Irish heritage, and his paternal grandfather was from Molise in southern Italy.
The award was given “for the cultural and artistic value of a career that has shaped the history of world cinema”, said the citation.
“Rome pays tribute to an extraordinary artist, capable of transcending genres and eras, with a versatility that has set a benchmark in contemporary cinema,” said Gualtieri.
“Robert De Niro is a benchmark, an absolute protagonist who has helped write the history of the silver screen, enchanting audiences with unforgettable performances,” continued the first citizen.
“Awarding him the Capitoline Wolf means thanking him for what he represents and for the deep bond that unites him to Rome, the capital of cinema and a source of inspiration for artists of every generation, which will always welcome him as a second home.”
“I thank the mayor and the city of Rome for this incredible honour,” said De Niro.
“Rome is more than a city, it’s a living work of art. Every street, every stone, every meal tells a story.
“To be recognised here, in a place that has given so much to the world—culture, cinema and beauty—is truly moving.
“My family has roots in Italy, so this recognition has personal meaning for me.
“I have always felt a deep connection to this country, its people, its passion and its respect for craftsmanship and creativity.
“These qualities are what inspire artists around the world.
“I receive this honour with immense gratitude—not only for myself, but for all those who continue to be touched by the spirit of Rome and what it represents,” De Niro concluded.
ANSA