Italian news agency ANSA reported that the ban is part of the raft of strict new regulations which recently became available to the city’s local police force.
The 18th-century steps, known as the Scalinata di Trinità dei Monti, are classified as a monument and UNESCO site, meaning that sitting or lying on them is prohibited.
Those caught in breach of the new ban face a fine of €250, which can rise to €400 if the steps are dirtied or damaged, according to ANSA.
In 2015, the steps underwent a €1.5-million restoration, funded by luxury jeweller Bulgari.
The baroque-style stairway, which comprises 136 travertine steps, connects Piazza di Spagna with the Trinità dei Monti church above.
Police have been spotted blowing whistles to order people up and off the steps this week, according to local media.
