The court also ordered Uber to cease operations of the UberLUX, UberSUV, UberX, UberXL, UberSELECT and UberVAN services, upholding taxi drivers’ claims that they constituted unfair competition.
Italy's taxi drivers have staged a series of strikes, some violent, against multinational ride-sharing service Uber.
Uber Italia said it would appeal the decision, claiming that it was based on a 25-year-old law which is outdated.
"Now the government cannot waste more time, but must decide whether it wants to remain anchored in the past, or to allow Italians to benefit from new technologies like Uber," the company said.
The US multinational said it was "stunned" at the court's decision, declaring that it rejects EU norms and values.
In its ruling, the court found that Uber had claimed an "undue advantage" over taxi drivers.
