SPAL said "it would like to thank the coach and the members of his staff for their work over the last few months, wishing them the best professional luck for the future of their careers".
The underperforming club has quickly appointed another member of the Azzurri's 2006 World Cup-winning squad, Massimo Oddo, to helm the club for the rest of the season.
The SPAL job was De Rossi's first coaching appointment.
The 39-year-old last played for Boca Juniors, where he only participated in five games after leaving Roma after 18 seasons in 2019, where he was known as 'Future Captain" behind icon and fellow World Cup winner Francesco Totti.
With the two in full flight, Roma won the league in 2001.
SPAL said Oddo's contract will run until the end of the current season, with an automatic renewal for another year if the team avoids relegation.
His backroom staff includes former Italy international Stefano Fiore.
ANSA
