The boys stormed into an emergency ward at the Loreto Mare Hospital to demand their friend receive urgent treatment.
The incident was reported on a Facebook page created by doctors in 2017 to highlight violence against health workers.
It is not clear if the teenagers had initially tried to call an ambulance.
“A group of boys entered the emergency room and took the crew by force... to board the ambulance,” the Facebook post by the group Nobody Touches Hippocrates reads.
“The vehicle arrived at the location and was immediately surrounded by a group of angry bystanders.
“Fearing the worst, the [doctors] made their way through the crowd and, to their surprise, found a 16-year-old boy with a knee sprain.”
According to the Facebook post, the ambulance staff explained to those at the scene that the boy’s injury was not serious, but were then threatened again for refusing to take him to hospital.
The incident was resolved after the boy received on-the-spot treatment.
On Monday, the founder and president of Nobody Touches Hippocrates, Manuel Ruggiero, suggested increasing security at some of Italy’s hospitals.
“Where it is not possible to have police in the emergency room, army units could be useful to stem the growing violence against health personnel,” he told Italian news agency ANSA.
The city of Naples has experienced at least five attacks on medical professionals in as many days, Italian media reported on Monday.
So far in 2020, two ambulances have been set on fire in the middle of city streets, in one case injuring a doctor.