The three are the CEO of La Linea, the company that operated the bus, and two city of Venice officials, the manager of the Traffic and Mobility department for the mainland area of the city and the manager of the Road Maintenance department, according to the report.
They reportedly face charges of vehicular homicide, multiple culpable homicides, causing serious or very serious road injuries and culpable personal harm.
Last week 21 people - nine Ukrainians, four Romanians, three Germans, two Portuguese, one Croatian, one South African and the Italian bus driver - died after a tourist bus travelling from the centre of Venice to a camping site plunged from an overpass above a railway line between the districts of Mestre and Marghera and burst into flames.
The reasons for the accident are unclear.
One hypothesis is that the driver made a bad turn while at the wheel.
However, doubts have also been raised about the state of the overpass and particularly the guardrail.
ANSA